Cedric Hardwicke

Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image)

Ralph Bellamy

Erik Ernst

Lionel Atwill

Dr. Bohmer

Bela Lugosi

Ygor

Evelyn Ankers

Elsa Frankenstein

Lon Chaney Jr.

The Frankenstein Monster

Janet Ann Gallow

Cloestine Hussman

Barton Yarborough

Dr. Kettering

Doris Lloyd

Martha the Housekeeper

Leyland Hodgson

Chief Constable

Olaf Hytten

Adolph Hussman

Holmes Herbert

Magistrate